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Scalable Fabrication of Stretchable, Dual Channel, Microfluidic Organ Chips
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Engineering Shelf-Stable Coating for Microfluidic Organ-on-a-Chip Using Bioinspired Catecholamine Polymers.

Sultan Khetani1,2,3, Kar Wey Yong1,2,4, Vinayaraj Ozhukil Kollath5

  • 1BioMEMS and Bioinspired Microfluidic Laboratory, Department of Mechanical and Manufacturing Engineering , University of Calgary , Calgary , Alberta T2N 1N4 , Canada.

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Summary

A new flow-based coating technique using bioinspired polymers creates stable, reliable microfluidic chips for organ-on-a-chip (OOC) studies. This method ensures long-term cell viability and functionality, simplifying OOC system development.

Keywords:
MALDI-ToF-MSbioinspired polymersorgan-on-a-chippolycatecholaminepolydopaminepolynorepinephrineshelf life

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Cell Biology

Background:

  • The conceptualization of body-on-a-chip technology has advanced human physiology studies in 3D culture.
  • Challenges persist in the stability, reliability, and preservation of microfluidic chips for organ-on-a-chip (OOC) applications.
  • Developing robust surface treatment techniques is crucial for simplifying OOC system creation and implementation.

Purpose of the Study:

  • To implement a novel flow-based coating technique using bioinspired polymers for creating reliable and reproducible microfluidic cell culture chips for OOC studies.
  • To evaluate the stability and functionality of microfluidic chips coated with polydopamine (PDA) and polynorepinephrine (PNE) under various conditions relevant to OOC applications.
  • To assess the long-term shelf life and performance of these coated chips for cell culture.

Main Methods:

  • Developed a flow-based coating technique using bioinspired catecholamine polymers (PDA and PNE) on polydimethylsiloxane microfluidic chips.
  • Conducted extensive surface characterizations and functional tests under diverse conditions (high temperature, various media, static/flow, long-term storage).
  • Assessed cell viability, phenotype, and functionality of cultured mouse cancer cells (CAD) and human endothelial cells (hCMEC/D3) on coated chips.

Main Results:

  • PDA- and PNE-coated microfluidic chips demonstrated high stability and reliability under various stress conditions.
  • Coated chips maintained excellent cell viability, phenotype, and functionality for both CAD and hCMEC/D3 cell lines.
  • CAD cells cultured on coated chips stored for up to 120 days showed sustained high viability.

Conclusions:

  • Flow-based polycatecholamine coating is an effective method for generating ready-to-use microfluidic OOC chips.
  • This technique offers long-term stability and reliability, facilitating the development of OOC systems for disease modeling and drug screening.
  • The developed method simplifies the process of creating robust microfluidic platforms for advanced physiological studies.