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Updated: Dec 30, 2025

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

Engineered minichromosomes in plants.

James A Birchler¹, Nathan C Swyers¹

¹Division of Biological Sciences, University of Missouri, 311 Tucker Hall, Columbia, MO, 65211-7400, USA.

Experimental Cell Research

|January 24, 2020

Summary

This summary is machine-generated.

Creating artificial chromosomes in plants is challenging due to epigenetic centromere function. Telomere-mediated truncation offers a viable method for engineering minichromosomes, with ongoing work to enhance their stability and utility.

Keywords:

Artificial chromosomes Gene stacking Maize Site specific recombinases Synthetic chromosomes Telomere-mediated truncation

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Area of Science:

Plant genetics
Molecular biology
Chromosomal engineering

Background:

Centromere function in plants is largely epigenetic, hindering artificial chromosome creation using centromeric DNA.
Centromeric histone removal complicates the use of plant centromere DNA for artificial chromosomes.

Purpose of the Study:

To describe methods for creating artificial chromosome platforms in plants.
To address challenges in plant artificial chromosome development, particularly centromere function and stability.

Main Methods:

Engineered minichromosomes produced via telomere-mediated chromosomal truncation.
Introduction of telomere repeats induces cleavage and transgene integration.
Truncation events documented in various plant species including maize, Arabidopsis, barley, rice, Brassica, and wheat.

Main Results:

Telomere-mediated truncation successfully generated engineered minichromosomes in multiple plant species.
Truncation of maize B chromosome and A chromosomes yielded minichromosomes.
Mitotic transmission of minichromosomes appears normal, but meiotic loss occurs.

Conclusions:

Telomere-mediated truncation is a foundational procedure for plant artificial chromosome development.
Current research focuses on enhancing minichromosome stability and functionality using gene stacking and editing.
Addressing meiotic instability is crucial for the practical application of plant artificial chromosomes.