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Published on: January 20, 2022
An improved and low-cost protocol for high-quality DNA isolation for the Chagas disease vectors
Daryl David Cruz1, Elizabeth Nava-García2, Elizabeth Arellano1
1Centro de Investigaciones en Biodiversidad y Conservación, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001 Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico.
Abstract:
An improved protocol for DNA extraction for the Chagas disease vectors is proposed based on modification to a low cost method described twenty years ago. Quality DNA and high molecular weight were obtained from all samples. NADH-4 gene was successfully amplified by PCR using the isolated DNA. The extraction protocol presented in this technical note is a fast, low-cost, and non-aggressive method to human health for obtaining genetic data from this group of epidemiological importance and potentially other insects.
