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The Probiotic BB12 Induces MicroRNAs Involved in Antigen Processing and Presentation in Porcine Monocyte-Derived
Marlene Bravo-Parra1, Marina Arenas-Padilla1, Valeria Brcenas-Preciado1
1Laboratory of Microbiology and Immunology, Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo A. C. (CIAD). Carretera Gustavo Astiazarán Rosas 46, Col. la Victoria, Hermosillo 83304, Sonora, Mexico.
Abstract:
MicroRNAs (miRNAs) mediate the regulation of gene expression. Several reports indicate that probiotics induce miRNA-mediated immunomodulation at different levels, such as cytokine production and the up-regulation of several markers related to antigen presentation in antigen-presenting cells. The objective of this work was to identify target genes of miRNAs that are involved in the processing and presentation of antigens in monocyte-derived dendritic cells (moDCs) stimulated with the probiotic Bifidobacterium animalis ssp. lactis BB12 (BB12). First, an in silico prediction analysis for a putative miRNA binding site within a given mRNA target was performed using RNAHybrid software with mature sequences of differentially expressed miRNAs retrieved from a Genbank data set that included BB12-stimulated and unstimulated porcine monocytes. From them, 23 genes resulted in targets of 19 miRNAs, highlighting miR-30b-3p, miR-671-5p, and miR-9858-5p, whose targets were costimulatory molecules, and were overexpressed (p < 0.05) in BB12-stimulated moDCs. The analysis of moDCs showed that the percentage of cells expressing SLA-DR+CD80+ decreased significantly (p = 0.0081) in BB12-stimulated moDCs; interleukin (IL)-10 production was unchanged at 6 h but increased after 24 h of culture in the presence of BB12 (p < 0.001). In summary, our results suggest that SLA-DR and CD80 can be down-regulated by miRNAs miR-30b-3p, miR-671-5p, and miR-9858-5p, while miR-671-5p targets IL-10.
Insights
Probiotics like Bifidobacterium animalis BB12 modulate immune responses by altering microRNAs (miRNAs). This study identified specific miRNAs that target genes involved in antigen presentation, suggesting a mechanism for probiotic-induced immunomodulation.
Area of Science:
- Immunology
- Molecular Biology
- Microbiology
Background:
- MicroRNAs (miRNAs) are key regulators of gene expression.
- Probiotics are known to induce immunomodulation through miRNA-mediated pathways.
- Antigen-presenting cells (APCs) play a crucial role in immune responses.
Purpose of the Study:
- To identify miRNA target genes involved in antigen processing and presentation.
- To investigate the effect of Bifidobacterium animalis ssp. lactis BB12 (BB12) on miRNA-mediated immune responses in dendritic cells.
Main Methods:
- In silico prediction analysis using RNAHybrid software to identify miRNA-mRNA interactions.
- Differential expression analysis of miRNAs in BB12-stimulated and unstimulated porcine monocytes.
- Flow cytometry to analyze SLA-DR and CD80 expression on monocyte-derived dendritic cells (moDCs).
- Interleukin-10 (IL-10) production measurement in moDCs.
Main Results:
- The study identified 19 miRNAs targeting 23 genes involved in antigen presentation.
- miR-30b-3p, miR-671-5p, and miR-9858-5p were highlighted for targeting costimulatory molecules.
- BB12 stimulation led to a significant decrease in SLA-DR+CD80+ moDCs.
- IL-10 production increased in BB12-stimulated moDCs after 24 hours.
Conclusions:
- Specific miRNAs, including miR-30b-3p, miR-671-5p, and miR-9858-5p, may down-regulate SLA-DR and CD80 expression.
- miR-671-5p was identified as a potential target of IL-10.
- These findings elucidate miRNA-mediated mechanisms underlying probiotic-induced immunomodulation.
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