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Stability of serum fructosamine during storage
1Central Laboratory, University Central Hospital of Turku, Finland.
Clinical Chemistry
|December 1, 1988
Summary
Serum fructosamine stability varies significantly during storage. Storing samples at -70°C minimizes pre-analytical variation, ensuring more reliable diabetic monitoring.
Area of Science:
- Clinical Chemistry
- Endocrinology
- Laboratory Medicine
Background:
- Serum fructosamine is a marker for glycemic control in diabetic patients.
- Accurate fructosamine measurement is crucial for effective diabetes management.
- Understanding sample stability during storage is essential for reliable diagnostic results.
Purpose of the Study:
- To evaluate the stability of serum fructosamine under different storage conditions.
- To determine the impact of prolonged storage at -20°C and -70°C on fructosamine levels.
- To identify factors influencing serum fructosamine degradation during storage.
Main Methods:
- Serum samples from 27 diabetic individuals were collected.
- Samples were divided into aliquots and stored at -20°C and -70°C for 2, 8, and 16 months.
- Fructosamine concentrations were measured after storage, and percentage changes were calculated.
Main Results:
- Significant variation in fructosamine levels was observed among individual specimens (-20% to 26.7%).
- Mean percentage changes across storage conditions ranged from -4.6% to 7.5%.
- Progressive in vitro glycation, particularly at -20°C, contributed to degradation.
Conclusions:
- Serum fructosamine exhibits considerable instability during prolonged storage, influenced by temperature.
- Storage at -70°C is preferable to minimize pre-analytical variation and in vitro glycation.
- Small changes in fructosamine concentrations after storage should be interpreted with caution.
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