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Poly(benzoxazine)s Modified with Osmium Complexes as a Class of Redox Polymers for Wiring of Enzymes to Electrode
Sabine Alsaoub1, Stefan Barwe1, Corina Andronescu2
1Analytical Chemistry-Center for Electrochemical Sciences (CES), Ruhr-Universität-Bochum, Universitätsstrasse 150, 44780 Bochum (Germany), Fax: (+49) 234 3214683.
Abstract:
Benzoxazine-based redox polymers bearing Os complexes are synthesized and used as an immobilization matrix for glucose oxidase (GOx) as a model system for a reagentless biosensor. The polymers are formed by electrochemically induced anodic polymerization of the corresponding benzoxazine monomers modified with Os complexes. The precursors are synthesized in a Mannich-type reaction between bisphenol A, formaldehyde, and the corresponding Os complexes or ligands, which contain free amino groups. The Os complexes are redox active within the polymer films, and thus, can be used as redox relays for the electron transfer between the electrode surface and the prosthetic group within the enzyme. Entrapment of GOx within the poly(benzoxazine) film is achieved successfully, as shown by the biocatalytic activity of the poly(benzoxazine)/GOx films upon the addition of glucose.
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