Physiological factors influencing the growth of skeletal muscle

The growth of muscle can be regulated by developmental changes or by alterations in hormone levels or in the rate or amount of work demanded. The mechanisms and structures involved in growth processes can be studied by controlling these factors. The models used are chicken anterior latissimus dorsi (ALD) muscle under the influence of overloading and rabbit tibialis anterior (TA) muscle under the influence of chronic nerve stimulation. Both models involve changes in the isoform of myosin that is expressed. Methods of study include quantitative ultrastructural analysis, immunofluorescence and in situ mRNA hybridization. In overloaded chick ALD fibres polysomes are nonuniformly distributed between the myofibrils and in a peripheral annulus even though subcellular concentrations of the new isoform are not found. In normal rabbit muscle the highest concentration of myosin mRNA detected by in situ hybridization is found in the subsarcolemmal zone. In stimulated TA polysomes are found between myofibrils. It appears that the myosin mRNA accumulates at specific cell locations before translation; then diffusion of isomyosin and rapid exchange into myofibrils follows. Therefore, regulation of growth may be possible at the transcriptional, translational and assembly stages.