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Related Experiment Video

Updated: Dec 30, 2025

Isolation and Transcriptome Analysis of Plant Cell Types

Isolation and Transcriptome Analysis of Plant Cell Types

Published on: April 7, 2023

Cell Type-Specific Transcriptomics in the Plant Embryo Using an Adapted INTACT Protocol.

Joakim Palovaara¹, Dolf Weijers²

¹Molecular Genetics, University of Bremen, Bremen, Germany. joakim@uni-bremen.de.

Methods in Molecular Biology (Clifton, N.J.)

|January 25, 2020

Summary

This summary is machine-generated.

Researchers developed a new method to study gene activity in plant embryos. This technique helps understand how different plant cell types develop from stem cells.

Keywords:

Cell type-specific Embryo Isolation Nucleus Transcriptome

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Last Updated: Dec 30, 2025

Isolation and Transcriptome Analysis of Plant Cell Types

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Published on: April 7, 2023

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Published on: June 7, 2013

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Laser-assisted Microdissection LAM as a Tool for Transcriptional Profiling of Individual Cell Types

Published on: May 10, 2016

Area of Science:

Plant biology
Developmental biology
Genomics

Background:

Cell differentiation is crucial for forming vascular plant tissues, with cell types specified early in embryonic development.
Understanding genome-wide transcriptional control during plant cell-type specification is limited.
Technical challenges in obtaining transcriptomes from inaccessible embryonic tissues hinder research.

Purpose of the Study:

To present an adapted INTACT (Isolation of Nuclei Tagged in Cytoplasm) protocol for generating cell-type specific transcriptomic profiles in the early Arabidopsis thaliana embryo.
To overcome technical hurdles in isolating nuclei and generating expression atlases from limited embryonic material.

Main Methods:

Adaptation of the INTACT system for isolating nuclei from various developmental stages of Arabidopsis thaliana embryos.
Generation of microarray-based expression profiles from isolated nuclei to create a cell-type specific expression atlas.

Main Results:

A detailed, step-by-step protocol for the adapted INTACT method is provided.
The study successfully generated transcriptomic profiles from specific cell types within the early plant embryo.
The protocol is optimized for limited starting material and diverse embryonic developmental stages.

Conclusions:

The adapted INTACT protocol provides a viable method for dissecting transcriptional control in plant embryogenesis.
This approach facilitates the creation of cell-type specific expression atlases, advancing the understanding of plant development.
The methodology is robust and adaptable for future studies on plant cell-type specification.