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Rapid preparation of the nicotinic acetylcholine receptor for crystallization in detergent solution
S Hertling-Jaweed1, G Bandini, A Müller-Fahrnow
1Institut für Biochemie, Freie Universität Berlin, Germany.
FEBS Letters
|December 5, 1988
Abstract:
A novel rapid purification method for the nicotinic acetylcholine receptor from Torpedo electric tissue was developed. It allows preparation of 10 mg quantities of pure and stabile receptor protein within 2 days. This protein is used for crystallization attempts. Conditions are described which reproducibly yield crystals.