Effects of concurrent training on irisin and fibronectin type-III domain containing 5 (FNDC5) expression in visceral...

Evidence suggests that myokines could have clinical implications for metabolic diseases such as type-2 diabetes.

We investigated the effects of concurrent training (CT) on irisin and fibronectin type-III domain containing five (FNDC5) expressions in visceral adipose tissue (VAT) in type-2 diabetic rats.

Eighteen male Wistar rats (ages four to eight weeks) became diabetic using nicotinamide and streptozotocin and were assigned to either a control (CON) or a CT group using a randomised block design. The CT group exercised on a motor-driven treadmill at 60 to 75 per cent of VO_2max (0% grade) for 10-40 min/day (aerobic training) and performed 10 climbs on a 1-meter ladder utilising weighted resistance of 30-100% of body mass (resistance exercise) for 5 days/week over 8 weeks. Forty-eight hours after the last training session, the VAT of rats was removed and washed. FNDC5-relative gene expression and irisin were measured by the reverse transcription polymerase chain reaction (RT-PCR) method and enzyme-linked immunosorbent assay (ELISA) kit. Additionally, insulin resistance and plasma insulin and glucose levels were determined.

Our findings revealed that CT significantly increased FNDC5-relative gene expression in the VAT of type-2 diabetic rats compared to controls. Furthermore, eight weeks of CT improved insulin resistance and insulin and glucose levels but did not significantly alter irisin levels in type-2 diabetic rats.

The results of this study demonstrated that CT increased FNDC5 mRNA and improved insulin resistance, insulin, and glucose levels. Also observed were increased trends (non-significant, p = .051) in irisin levels. Hence, CT may play a role in attenuating metabolic disorders such as obesity and type-2 diabetes.