Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Intermolecular Forces03:13

Intermolecular Forces

68.3K
Atoms and molecules interact through bonds (or forces): intramolecular and intermolecular. The forces are electrostatic as they arise from interactions (attractive or repulsive) between charged species (permanent, partial, or temporary charges) and exist with varying strengths between ions, polar, nonpolar, and neutral molecules. The different types of intermolecular forces are ion–dipole, dipole–dipole, hydrogen bonds, and dispersion; among these, dipole–dipole, hydrogen...
68.3K
Ions as Acids and Bases02:54

Ions as Acids and Bases

25.9K
Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
25.9K
Polyprotic Acids03:38

Polyprotic Acids

31.5K
Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:
31.5K
Aqueous Solutions and Heats of Hydration02:42

Aqueous Solutions and Heats of Hydration

17.2K
Water and other polar molecules are attracted to ions. The electrostatic attraction between an ion and a molecule with a dipole is called an ion-dipole attraction. These attractions play an important role in the dissolution of ionic compounds in water.
When ionic compounds dissolve in water, the ions in the solid separate and disperse uniformly throughout the solution because water molecules surround and solvate the ions, reducing the strong electrostatic forces between them. This process...
17.2K
Amino acids03:42

Amino acids

103.3K
Amino acids are the monomers that comprise proteins. Each amino acid has the same fundamental structure, which consists of a central carbon atom, or the alpha (α) carbon, bonded to an amino group (NH2), a carboxyl group (COOH), and to a hydrogen atom. Every amino acid also has another atom or group of atoms bonded to the central atom known as the R group. There are 20 common amino acids present in proteins, each with a different R group. Variation in the amino acid sequence is responsible for...
103.3K
¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

1.2K
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
1.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reducing Ice Adhesion to Polyelectrolyte Surfaces by Counterion-Mediated Nonfrozen Hydration Water.

ACS applied materials & interfaces·2024
Same author

Orientational Behavior and Vibrational Response of Glycine at Aqueous Interfaces.

The journal of physical chemistry letters·2024
Same author

Calcite Surfaces Modified with Carboxylic Acids (C<sub>2</sub> to C<sub>18</sub>): Layer Organization, Wettability, Stability, and Molecular Structural Properties.

Langmuir : the ACS journal of surfaces and colloids·2023
Same author

Observation of a Two-Dimensional Hydrophobic Collapse at the Surface of Water Using Heterodyne-Detected Surface Sum-Frequency Generation.

The journal of physical chemistry letters·2023
Same author

Ice breakloose friction.

The Journal of chemical physics·2023
Same author

Observation of Strong Synergy in the Interfacial Water Response of Binary Ionic and Nonionic Surfactant Mixtures.

The journal of physical chemistry letters·2022
Same journal

PCSK5 promotes angiogenesis and cardiac repair after myocardial infarction.

Nature communications·2026
Same journal

PfApiAT2 is a proline transporter essential for the transmission of Plasmodium falciparum by the mosquito vector.

Nature communications·2026
Same journal

Transient distortions of the South Atlantic Anomaly radiation environments driven by electric fields.

Nature communications·2026
Same journal

Structural basis of the regulation by CDK11 kinase of early spliceosome activation and evidence for its proofreading by DHX15 helicase.

Nature communications·2026
Same journal

Structural and mechanistic insights into primer synthesis initiation by DNA primase.

Nature communications·2026
Same journal

Changes in heritability and shared environmentality of educational attainment across twentieth-century Norway.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Dec 30, 2025

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid
08:54

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid

Published on: January 25, 2020

5.9K

Identifying Eigen-like hydrated protons at negatively charged interfaces.

Eric Tyrode1, Sanghamitra Sengupta2, Adrien Sthoer2

  • 1Department of Chemistry, KTH Royal Institute of Technology, SE-10044, Stockholm, Sweden. tyrode@kth.se.

Nature Communications
|January 26, 2020
PubMed
Summary
This summary is machine-generated.

Researchers identified the molecular structure of hydrated protons (H3O+) at charged interfaces using vibrational spectroscopy. This breakthrough allows for molecular-level tracking of proton behavior in biologically relevant conditions.

More Related Videos

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
09:48

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

Published on: February 15, 2016

8.7K
Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

9.3K

Related Experiment Videos

Last Updated: Dec 30, 2025

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid
08:54

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid

Published on: January 25, 2020

5.9K
In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS
09:48

In Situ Characterization of Hydrated Proteins in Water by SALVI and ToF-SIMS

Published on: February 15, 2016

8.7K
Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy
10:28

Probing the Structure and Dynamics of Interfacial Water with Scanning Tunneling Microscopy and Spectroscopy

Published on: May 27, 2018

9.3K

Area of Science:

  • Physical Chemistry
  • Surface Science
  • Spectroscopy

Background:

  • The molecular structure of hydrogen ions in solution is crucial for many processes but remains poorly understood due to diffuse vibrational signatures.
  • Understanding hydrated protons is vital for biological, chemical, and physical sciences.

Purpose of the Study:

  • To identify and characterize the molecular structure of hydrated protons (H3O+) at charged interfaces.
  • To develop a method for tracking proton behavior at biologically relevant pH.

Main Methods:

  • Utilized vibrational sum frequency spectroscopy (VSFS), a surface-specific technique.
  • Investigated negatively charged interfaces in a biologically compatible pH range.
  • Performed isotopic substitution experiments (H3O+ to D3O+) to confirm spectral assignments.

Main Results:

  • Successfully identified a resolved spectral feature linked to the H3O+ core in an Eigen-like species at ~2540 cm-1.
  • Observed a spectral shift to ~1875 cm-1 upon isotopic substitution to D3O+.
  • Demonstrated the ability to detect hydrated protons at charged interfaces.

Conclusions:

  • Vibrational sum frequency spectroscopy can readily identify hydrated protons at charged interfaces.
  • The findings provide a molecular perspective for understanding proton behavior at interfaces.
  • This technique opens possibilities for tracking proton dynamics in complex systems.