Related Experiment Video
Updated: Dec 30, 2025
09:14
Isolation of Papillary and Reticular Fibroblasts from Human Skin by Fluorescence-activated Cell Sorting
Published on: May 7, 2019
16.7K
YAP/TAZ direct commitment and maturation of lymph node fibroblastic reticular cells
Sung Yong Choi1,2, Hosung Bae3, Sun-Hye Jeong4
1Center for Vascular Research, Institute for Basic Science (IBS), Daejeon, 34141, Republic of Korea.
Nature Communications
|January 26, 2020
Summary
Hippo signaling effectors YAP/TAZ control the development of fibroblastic reticular cells (FRCs) in lymph nodes (LNs). Dysregulation of YAP/TAZ impacts LN structure and fibrosis, offering insights into immune system organization.
Area of Science:
- Immunology
- Cell Biology
- Developmental Biology
Background:
- Fibroblastic reticular cells (FRCs) are crucial myofibroblasts in lymphoid organs, essential for lymph node (LN) structure and function.
- FRC maturation is vital for maintaining the immunological integrity and organization of LNs.
Purpose of the Study:
- To investigate the role of YAP and TAZ (YAP/TAZ), key Hippo signaling pathway components, in regulating FRC commitment and maturation.
- To elucidate the molecular mechanisms by which YAP/TAZ influence FRC development and LN homeostasis.
Main Methods:
- Utilized genetic manipulation to selectively deplete or hyperactivate YAP/TAZ in FRCs within a mouse model.
- Analyzed the impact of YAP/TAZ modulation on FRC proliferation, differentiation, LN structure, and fibrotic changes.
- Investigated molecular interactions involving YAP/TAZ, p52, and lymphotoxin-β receptor (LTβR) signaling.
Main Results:
- Selective YAP/TAZ depletion in FRCs led to impaired FRC growth, differentiation, and compromised LN structural organization.
- Hyperactivation of YAP/TAZ in FRCs resulted in enhanced myofibroblastic features and exacerbated LN fibrosis.
- Mechanistically, YAP/TAZ interaction with p52 promotes chemokine expression for FRC lineage commitment, while LTβR signaling suppresses YAP/TAZ activity during FRC maturation.
Conclusions:
- YAP/TAZ are critical regulators of both the commitment and maturation processes of fibroblastic reticular cells.
- These findings provide a deeper understanding of FRC biology and their role in LN pathophysiology.
- The study highlights YAP/TAZ as potential therapeutic targets for modulating FRC-mediated diseases.
Related Concept Videos
01:16Primary Lymphoid Organs
14.4K
Primary lymphoid organs are pivotal in the formation, development, and maturation of lymphocytes, the white blood cells that serve as the backbone of our immune system. This crucial function underscores their fundamental role in maintaining our overall health and immunity. The two primary lymphoid organs of prime importance are the red bone marrow and the thymus.
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...
14.4K
01:18Lymphoid Cells and Tissues
2.7K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
2.7K
01:23Cells of the Adaptive Immune Response
8.2K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
8.2K
01:23Detailed Structure and Function of Lymph Nodes
4.1K
Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...
4.1K
01:15Development of the Lymphatic System
1.8K
The development of lymphatic tissues and vessels in embryonic life begins around the fifth week. These structures originate from the mesoderm layer, with lymph sacs emerging from developing veins.
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
1.8K
01:15Secondary Lymphoid Organs
8.7K
Secondary organs, including lymph nodes, the spleen, and mucosa-associated lymphoid tissue (MALT), work harmoniously to protect us from disease and infection.
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
The spleen is a vital organ in the lymphatic system, nestled in the upper left side of the abdomen. It is composed of two primary regions: the red pulp and the white pulp, each having distinct functions. The red pulp performs a significant role in blood filtration. It efficiently purges the blood of old or damaged red blood cells and...
8.7K