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Human organ culture techniques for the detection and evaluation of genotoxic agents
W P Watson1, R J Smith, K R Huckle
1Shell Research Ltd, Sittingbourne Research Centre, Kent, UK.
IARC Scientific Publications
|January 1, 1988
Abstract:
In order to obtain information on the genotoxic metabolism of carcinogens in human skin in vivo, model in-vitro systems have been developed to mimic in-vivo metabolism qualitatively. Direct labelling (3H and 14C) and 32P-postlabelling analyses of benzo[alpha]pyrene (BP)-DNA adducts in human skin explants, CD1 mouse explants and CD1 mouse skin in vivo have thus allowed comparisons of the genotoxic metabolism of BP in mouse and human skin.