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Gga-miR-30d regulates infectious bronchitis virus infection by targeting USP47 in HD11 cells

Hao Li1, Jianan Li1, Yaru Zhai1

  • 1Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610065, Sichuan, PR China.

Microbial Pathogenesis
|January 27, 2020
PubMed

Insights

Avian infectious bronchitis virus (IBV) replication is regulated by gga-miR-30d. Overexpressing this microRNA inhibits IBV, while low levels promote its replication, impacting poultry health.

Area of Science:

  • * Virology
  • * Molecular Biology
  • * Poultry Science

Background:

  • * Avian infectious bronchitis virus (IBV) causes significant economic losses in the global poultry industry.
  • * MicroRNAs (miRNAs) are crucial intracellular regulators involved in viral infections.
  • * Previous research indicated IBV infection down-regulates gga-miR-30d expression in chicken kidneys.

Purpose of the Study:

  • * To investigate the role of gga-miR-30d in the in vitro infection process of IBV in HD11 cell line.
  • * To determine how gga-miR-30d levels affect IBV replication.
  • * To identify potential targets of gga-miR-30d involved in IBV infection.

Main Methods:

  • * Transfection of HD11 cell line with gga-miR-30d mimics and inhibitors.
  • * Measurement of IBV replication following gga-miR-30d manipulation.
  • * Dual-luciferase reporter assays to identify gene targets of gga-miR-30d.

Main Results:

  • * Overexpression of gga-miR-30d significantly inhibited IBV replication in HD11 cells.
  • * Reduced expression of gga-miR-30d promoted IBV replication.
  • * Ubiquitin-specific protease 47 (USP47) was identified as a direct target of gga-miR-30d.

Conclusions:

  • * gga-miR-30d plays a regulatory role in controlling IBV replication.
  • * This study demonstrates a novel mechanism where miRNAs regulate viral replication by targeting deubiquitinating enzymes (DUBs).
  • * Findings provide insights into host-virus interactions and potential therapeutic targets for IBV infections.
Keywords:
HD11IBVUSP47miR-gga-30d

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