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Mismatch Repair

Mismatch Repair

Mismatch Repair

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

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Updated: Dec 30, 2025

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

Published on: June 8, 2020

DNA Mismatch Repair Deficiency Detection in Colorectal Cancer by a New Microsatellite Instability Analysis System.

Shafei Wu¹, Xiaoding Liu¹, Jing Wang¹

¹Department of Pathology, Molecular Pathology Research Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences (CAMS), Beijing, 100730, China.

Interdisciplinary Sciences, Computational Life Sciences

|January 27, 2020

Summary

The ProDx® MSI analysis system accurately classifies colorectal cancer (CRC) microsatellite instability (MSI) status. This new method identifies more MSI-High CRC cases than previous systems, improving diagnostic capabilities.

Keywords:

Colorectal cancer Microsatellite instability Mismatch repair deficiency

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Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

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Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter

Published on: March 31, 2022

Area of Science:

Oncology
Molecular Diagnostics
Genetics

Background:

Microsatellite instability (MSI) is a key biomarker in colorectal cancer (CRC).
Accurate MSI classification is crucial for understanding CRC phenotypes and guiding treatment.
Existing MSI analysis methods may have limitations in challenging cases.

Purpose of the Study:

To evaluate the effectiveness of the ProDx® MSI analysis system for classifying MSI in CRC.
To compare the ProDx® MSI system with established methods like Promega MSI analysis System 1.2 and the NCI panel.

Main Methods:

Compared MSI profiles of 97 CRC FFPE samples using ProDx® MSI, Promega MSI analysis System 1.2, and NCI panel.
Validated results with immunohistochemistry (IHC) for mismatch repair (MMR) protein expression.
Utilized next-generation sequencing for discordant results.

Main Results:

ProDx® MSI identified 3 additional MSI-High CRC cases missed by other systems.
ProDx® MSI demonstrated higher concordance with IHC (99.0%) compared to Promega (96.9%) and NCI (95.9%).
Achieved 100% sensitivity and 98.4% specificity for MSI status determination.

Conclusions:

ProDx® MSI system accurately classifies colorectal cancer MSI status.
This system reveals more MSI-High cases in CRC than previous methods.
The ProDx® MSI system offers improved diagnostic potential for MSI analysis in CRC.