Cdk5 phosphorylates Ca_V1.3 channels and regulates GABA_A-mediated miniature inhibitory post-synaptic currents in...

Neurotransmission is one of the most important processes in neuronal communication and depends largely on Ca²⁺ entering synaptic terminals through voltage-gated Ca²⁺ (Ca_V) channels. Although the contribution of L-type Ca_V channels in neurotransmission has not been unambiguously established, increasing evidence suggests a role for these proteins in noradrenaline, dopamine, and GABA release. Here we report the regulation of L-type channels by Cdk5, and its possible effect on GABA release in the substantia nigra pars reticulata (SNpr). Using patch-clamp electrophysiology, we show that Cdk5 inhibition by Olomoucine significantly increases current density through Ca_V1.3 (L-type) channels heterologously expressed in HEK293 cells. Likewise, in vitro phosphorylation showed that Cdk5 phosphorylates residue S1947 in the C-terminal region of the pore-forming subunit of Ca_V1.3 channels. Consistent with this, the mutation of serine into alanine (S1947A) prevented the regulation of Cdk5 on Ca_V1.3 channel activity. Our data also revealed that the inhibition of Cdk5 increased the frequency of high K⁺-evoked miniature inhibitory postsynaptic currents in rat SNpr neurons, acting on L-type channels. These results unveil a novel regulatory mechanism of GABA release in the SNpr that involves a direct action of Cdk5 on L-type channels.