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Erythrocyte aggregation in iohexol and other nonionic media.

M B Zucker1, E A Mauss

  • 1Department of Pathology, New York University Medical Center, NY 10016.

Investigative Radiology
|November 1, 1988
PubMed
Summary

Erythrocytes (red blood cells) rapidly aggregate in nonionic solutions like glucose or iohexol, particularly at low pH. This aggregation is reversible and preventable with ionic solutes or near-neutral pH.

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Area of Science:

  • Biochemistry
  • Hematology
  • Medical Imaging

Background:

  • Erythrocyte aggregation is a critical factor in blood rheology and can be influenced by various physiological and non-physiological conditions.
  • Nonionic substances, such as glucose and contrast media, are frequently encountered in clinical settings and can interact with blood components.

Purpose of the Study:

  • To investigate the aggregation behavior of erythrocytes in the presence of nonionic solutes like glucose and iohexol.
  • To determine the influence of pH and ionic strength on nonionic-induced erythrocyte aggregation.

Main Methods:

  • Erythrocyte aggregation was induced using solutions of glucose and iohexol.
  • The effect of varying pH levels (e.g., 6.8 and 7.4) on aggregation was assessed.
  • The role of ionic solutes (e.g., NaCl) in modulating aggregation was examined.

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Main Results:

  • Erythrocytes exhibited rapid aggregation in nonionic solutions (glucose, iohexol), especially at reduced pH (6.8).
  • The observed aggregates were distinct from blood clots, lacking fibrin and rapidly dispersing in ionic media.
  • Aggregation was significantly reduced by the addition of low concentrations of ionic solutes (NaCl) or by maintaining a pH closer to 7.4.

Conclusions:

  • Nonionic solutes can induce rapid, reversible erythrocyte aggregation, particularly under acidic conditions.
  • pH and ionic environment are crucial factors modulating erythrocyte aggregation in nonionic solutions.
  • Understanding these aggregation dynamics is important for applications involving contrast media and blood handling.