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LncRNA MCM3AP-AS1 inhibits cell proliferation in cervical squamous cell carcinoma by down-regulating miRNA-93

Liu Lan1, Zhishan Liang2, Yingxi Zhao1

  • 1Department of Radiotheragy, The Second Affiliated Hospital of Guangxi University of Science and Technology, Liuzhou, Guangxi 545005, P.R. China.

Bioscience Reports
|January 28, 2020
PubMed
Abstract

Insights

MCM3AP-AS1 is downregulated in cervical cancer and inhibits cell proliferation by reducing miR-93. This suggests MCM3AP-AS1 acts as a tumor suppressor in CSCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MCM3AP-AS1 is an oncogenic lncRNA in some cancers.
  • MCM3AP-AS1 is downregulated in cervical squamous cell carcinoma (CSCC).

Purpose of the Study:

  • Investigate the role of MCM3AP-AS1 in CSCC.
  • Determine the relationship between MCM3AP-AS1 and miR-93 in CSCC.

Main Methods:

  • Analyzed TCGA dataset for MCM3AP-AS1 expression.
  • Used RT-qPCR and Methylation Specific PCR (MSP) to assess gene expression and methylation.
  • Performed cell transfections and CCK-8 assays to evaluate gene interactions and cell proliferation.

Main Results:

  • MCM3AP-AS1 is downregulated in CSCC and linked to poor survival.
  • MCM3AP-AS1 inversely correlates with miR-93; it downregulates miR-93 expression and upregulates its methylation.
  • Overexpression of MCM3AP-AS1 reduces CSCC cell proliferation, while silencing increases it.

Conclusions:

  • MCM3AP-AS1 acts as a tumor suppressor in CSCC.
  • MCM3AP-AS1 inhibits cell proliferation by downregulating miR-93.
Keywords:
MCM3AP-AS1cervical squamous cell carcinomamiR-93proliferationsurvival

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