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Related Experiment Video

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The Superficial Inferior Epigastric Artery Axial Flap to Study Ischemic Preconditioning Effects in a Rat Model
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Does ischemic preconditioning increase flap survival by ADORA2B receptor activation?

Pinar Ulker1, Ozlenen Ozkan2, Matteo Amoroso3

  • 1Department of Physiology, Akdeniz University, Antalya, Turkey.

Clinical Hemorheology and Microcirculation
|January 28, 2020
PubMed
Summary

Ischemic preconditioning protects tissues by activating adenosine receptors (ADORA2BR). This study shows ADORA2BR agonists may prevent ischemia/reperfusion injury in flap surgery.

Keywords:
ADORA 2BAdenosineflap survivalischemiareperfusion

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Area of Science:

  • Biomedical Science
  • Surgical Research
  • Pharmacology

Background:

  • Ischemic preconditioning (IPC) enhances tissue tolerance to ischemia.
  • Adenosine-mediated ADORA2B receptor (ADORA2BR) activation is a key IPC mechanism.
  • The role of ADORA2BR in flap surgery ischemia/reperfusion (I/R) injury is unexplored.

Purpose of the Study:

  • Investigate adenosine and ADORA2BR activation in IPC-mediated tissue protection.
  • Evaluate the therapeutic potential of ADORA2BR agonists in flap surgery I/R models.

Main Methods:

  • An epigastric flap model with 6-hour ischemia and 6-day reperfusion was used.
  • Animals were grouped to receive IPC, CD73 inhibition, adenosine, ADORA2BR antagonist, or ADORA2BR agonist.
  • Tissue survival was assessed macroscopically and histologically after reperfusion.

Main Results:

  • IPC increased CD73 expression and adenosine levels, enhancing flap survival.
  • CD73 inhibition blocked IPC's protective effect.
  • Adenosine and ADORA2BR agonists improved flap survival, while antagonists reduced it.

Conclusions:

  • CD73-dependent adenosine generation and ADORA2BR signaling mediate IPC tissue protection.
  • ADORA2BR agonists show promise as a preventive therapy for I/R injury in flap surgery.