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Investigating Memory Updating in Mice Using the Objects in Updated Locations Task.

Destiny S Wright1, Kasuni K Bodinayake1, Janine L Kwapis1

  • 1Department of Biology, Center for Molecular Investigation of Neurological Disorders (CMIND), Pennsylvania State University, University Park, Pennsylvania.

Current Protocols in Neuroscience
|January 28, 2020
PubMed
Summary

Researchers developed a new task to study how the aging brain updates memories. This Objects in Updated Locations (OUL) task assesses memory for original and new object locations in a single session.

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Old mice fail to integrate a memory update into an existing hippocampal engram.

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HDAC3 Serine 424 Phospho-mimic and Phospho-null Mutants Bidirectionally Modulate Long-Term Memory Formation and Synaptic Plasticity in the Adult and Aging Mouse Brain.

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Area of Science:

  • Neuroscience
  • Cognitive Science
  • Animal Behavior

Background:

  • Laboratory memory studies often use `de novo` experiences, unlike real-world memory updating.
  • The aging brain frequently modifies existing memories, necessitating research into memory updating mechanisms.

Purpose of the Study:

  • To introduce a novel behavioral paradigm, the Objects in Updated Locations (OUL) task, for studying memory updating.
  • To assess the OUL task's efficacy in evaluating both original and updated spatial memories in a single session.
  • To provide a low-stress method for studying memory updating in aging animals.

Main Methods:

  • The OUL task involves animals learning object locations in a familiar context.
  • Memory is updated by relocating one object, followed by a test session with multiple object copies.
Keywords:
aginghippocampusobject memoryreconsolidationupdating

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  • Exploration patterns in the test session reveal memory for original and updated locations.

    • Main Results:

    • The OUL task allows for the assessment of both original and updated spatial memories within one session.
    • Incidental learning in the OUL task minimizes stress, beneficial for aging animal studies.
    • The paradigm facilitates inferences about memory retention and updating.

    Conclusions:

    • The Objects in Updated Locations (OUL) task is a valuable tool for investigating memory updating.
    • This paradigm can offer new insights into the neural and molecular mechanisms of memory modification.
    • OUL supports research on how aging affects the ability to update memories.