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Related Experiment Video

Updated: Dec 30, 2025

Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Visual detection for nucleic acid-based techniques as potential on-site detection methods. A review.

Mengyao Zhang1, Jing Ye2, Jin-Song He3

  • 1College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.

Analytica Chimica Acta
|January 28, 2020
PubMed
Summary

Nucleic acid detection offers high sensitivity through amplification. This review focuses on advanced visual detection methods for simple, rapid, and instrument-free on-site diagnostics.

Keywords:
Colorimetric dyeIsothermal amplificationLateral flow dipstickNucleic acidOn-site detectionVisual detection

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Molecular Diagnostics

Background:

  • Nucleic acid-based techniques are crucial for sensitive detection in fields like disease diagnosis and food safety.
  • These methods involve nucleic acid extraction, amplification, and product detection, with detection being critical for direct result interpretation.
  • Current trends emphasize simple, rapid, and instrument-free detection, necessitating visual read-outs.

Purpose of the Study:

  • To review and discuss advanced visual detection methods for nucleic acid-based techniques.
  • To highlight the importance of the detection step in nucleic acid analysis.
  • To promote the application of visual detection for on-site testing.

Main Methods:

  • Literature review of advanced visual detection techniques for nucleic acid amplification.
  • Discussion of methods enabling naked-eye result interpretation.
  • Analysis of techniques suitable for simple, rapid, and instrument-free applications.

Main Results:

  • Summary of various advanced visual detection strategies for nucleic acid amplification products.
  • Identification of methods that allow for direct, visible results without instrumentation.
  • Assessment of the potential for these methods in on-site diagnostic scenarios.

Conclusions:

  • Advanced visual detection methods are essential for the trend towards simple, rapid, and instrument-free nucleic acid testing.
  • These techniques hold significant promise for enabling on-site detection in diverse fields.
  • Further development and application of visual detection are encouraged for accessible diagnostics.