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A new aptamer/black phosphorous interdigital electrode for malachite green detection.
Lidong Wu1, Zhiyuan Xu2, Qingyi Meng2
1Key Laboratory of Control of Quality and Safety for Aquatic Products, Chinese Academy of Fishery Sciences, Beijing, 100141, China.
Analytica Chimica Acta
|January 28, 2020
Summary
Researchers developed a novel biosensor using functionalized black phosphorus (BP) and cysteine (CYS) to detect Malachite Green (MG). This highly sensitive method offers rapid and selective MG determination, crucial for environmental and health monitoring.
Area of Science:
- Electrochemistry
- Materials Science
- Environmental Science
Background:
- Malachite Green (MG) is a toxic dye affecting immune and reproductive systems.
- Rapid, sensitive, and selective detection methods for MG are essential.
- Black phosphorus (BP) offers high charge-carrier mobility and adsorption capacity for cationic dyes like MG, making it a promising sensing platform.
- BP's susceptibility to degradation in humid conditions limits its practical application.
Purpose of the Study:
- To develop a stable and highly sensitive biosensor for Malachite Green (MG) detection.
- To overcome the degradation limitations of black phosphorus (BP) in sensing applications.
- To functionalize BP with cysteine (CYS) for enhanced stability and selectivity.
Main Methods:
- Functionalization of black phosphorus (BP) with cysteine (CYS) to prevent degradation.
- Fabrication of a silicon interdigital electrode incorporating the CYS-functionalized BP and an aptamer.
- Electrochemical detection of Malachite Green (MG) using the fabricated biosensor.
Main Results:
- The CYS-functionalized BP prevented BP degradation, enhancing sensor stability.
- The developed biosensor demonstrated high sensitivity and selectivity towards MG.
- A lowest detection limit of 0.3 ng L⁻¹ for MG was achieved with the BP-based interdigital electrode.
Conclusions:
- Cysteine (CYS) effectively protects black phosphorus (BP) from oxidation and degradation.
- The novel CYS-functionalized BP-aptamer biosensor provides a highly sensitive and selective platform for Malachite Green (MG) detection.
- This work presents a new strategy for large-scale, on-site MG monitoring biosensor development.
