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Developmental validation of the monSTR identity panel, a forensic STR multiplex assay for massively parallel
Janine Silvery1, Sebastian Ganschow2, Peter Wiegand3
1LABCON-OWL Analytik, Forschung und Consulting GmbH, Siemensstraße 40, 32105 Bad Salzuflen, Germany; Institute of Legal Medicine, University Hospital of Ulm, Albert-Einstein-Allee 23, 89081 Ulm, Germany.
Forensic Science International. Genetics
|January 28, 2020
Summary
The monSTR forensic identity panel offers high-fidelity STR genotyping for DNA analysis. This validated tool ensures accurate results for forensic casework using massively parallel sequencing.
Area of Science:
- Forensic Science
- Genetics
- Molecular Biology
Background:
- The monSTR 21-plex STR panel is designed for high-fidelity forensic genotyping.
- Validation is crucial for forensic DNA analysis tools according to SWGDAM guidelines.
Purpose of the Study:
- To validate the performance of the monSTR 21-plex STR panel for forensic applications.
- To assess concordance, sensitivity, mixture analysis, and reproducibility of the panel.
Main Methods:
- Validation according to SWGDAM guidelines.
- Assessed concordance with CE-based methods using NIST and GEDNAP samples.
- Evaluated sensitivity, mixture analysis (1:49), repeatability, and reproducibility.
- Utilized the toaSTR software for sequence data analysis.
Main Results:
- Full concordance with CE-based methods for reference and proficiency samples.
- High precision demonstrated through inter-run and intra-run variation experiments.
- Successful full profiles obtained with 62.5 pg DNA input.
- Accurate allele calling (76.4%) with 7.8 pg DNA input.
- Accurate resolution of minor contributor alleles (94.4%) in low-template mixtures.
Conclusions:
- The monSTR panel meets validation requirements for forensic STR genotyping.
- The panel demonstrates high accuracy, sensitivity, and precision for massively parallel sequencing.
- monSTR is a validated tool for reliable forensic DNA analysis.
