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Updated: Dec 30, 2025

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Generation and characterization of a human iPSC line derived from congenital clubfoot amniotic fluid cells

Jing Wang1, Yazhou Cui2, Kaixuan Xing2

  • 1School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; Shandong Medicinal Biotechnology Center, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan, Shandong, China; Key Laboratory for Biotech-Drugs of National Health Commission, Ji'nan, Shandong, China.

Stem Cell Research
|January 28, 2020
PubMed

Insights

A new human induced pluripotent stem cell line was created from amniotic fluid cells of a fetus with clubfoot. This cellular model offers a valuable resource for investigating clubfoot pathology.

Area of Science:

  • Stem cell biology
  • Developmental biology
  • Genetics

Background:

  • Clubfoot etiology remains unclear, hindering effective treatment development.
  • Human induced pluripotent stem cells (iPSCs) offer a promising in vitro model for studying congenital disorders.
  • Amniotic fluid-derived cells (AFDCs) are a viable source for iPSC generation.

Observation:

  • A novel human induced pluripotent stem cell (iPSC) line, SMBCi002-A, was successfully generated from amniocytes.
  • The iPSC line was derived from a female fetus diagnosed with clubfoot.
  • A non-integrative episomal plasmid strategy was employed for cell reprogramming, utilizing factors OCT4, SOX2, KLF4, MYC, LIN28, and EBNA1.

Findings:

  • Comprehensive validation assays confirmed the pluripotency of the SMBCi002-A iPSC line.
  • The established iPSC line demonstrated robust differentiation potential across multiple lineages.
  • Genetic stability assessments indicated the integrity of the reprogrammed cells.

Implications:

  • The SMBCi002-A iPSC line serves as a critical in vitro model for clubfoot research.
  • This cellular resource facilitates the investigation of clubfoot's underlying mechanisms and pathology.
  • Understanding clubfoot pathogenesis can pave the way for novel therapeutic strategies.

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