Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of Vero cells.

S K Swanson1, S J Mento, C Weeks-Levy

  • 1Lederle Laboratories Division, American Cyanamid Company, Pearl River, New York 10965.

Journal of Biological Standardization
|October 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Endoscopic removal of a bladder calculus via flexible sigmoidoscopy.

Endoscopy·2007
Same author

Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits.

Biochemical Society transactions·2004
Same author

Chromosomal localization of three vacuolar-H+ -ATPase 16 kDa subunit (ATP6V0C) genes in the murine genome.

Cytogenetic and genome research·2002
Same author

Comparison of I-125 sources used for permanent interstitial implants.

Medical physics·2001
Same author

Empirical dosimetric characterization of model I125-SL 125iodine brachytherapy source in phantom.

Medical physics·2001
Same author

Fitting and benchmarking of dosimetry data for new brachytherapy sources.

Medical physics·2000
Same journal

Monoclonal antibodies for therapy, prevention and in vivo diagnosis of human disease. International Association of Biological Standardization Symposium.

Journal of biological standardization·1989
Same journal

Salmonella typhi vaccine strain in vitro; low infectivity in human cell line U937.

Journal of biological standardization·1989
Same journal

[Study of diphtheria and tetanus anatoxins by electrofocusing on an agarose gel].

Journal of biological standardization·1989
Same journal

Preclinical investigations of the safety, immunogenicity and efficacy of a purified, inactivated tick-borne encephalitis vaccine.

Journal of biological standardization·1989
Same journal

In vitro induction of a diphtheria toxoid specific antibody response in human peripheral blood lymphocytes cultivated in the presence of diphtheria toxoid.

Journal of biological standardization·1989
Same journal

Standardization of an enzyme immunoassay for the in vitro potency assay of inactivated tissue culture rabies vaccines: determination of the rabies virus glycoprotein with polyclonal antisera.

Journal of biological standardization·1989
See all related articles

Vero cells, used for biological production, were tested and found to be free of retroviruses. These monkey kidney cells also showed no signs of tumor formation in mice, confirming their safety for biopharmaceutical manufacturing.

Area of Science:

  • * Cell line characterization and safety assessment for biopharmaceutical production.
  • * Virology and tumorigenicity testing of mammalian cell lines.

Background:

  • * Regulatory guidelines require biological production cell lines to be free of infectious agents.
  • * Cell lines must be characterized for cytogenetic properties and tumorigenicity.
  • * Vero cells, derived from African green monkey kidney, are widely used in biological manufacturing.

Purpose of the Study:

  • * To assess the presence of retroviruses in Vero cells.
  • * To evaluate the tumorigenic potential of Vero cells.
  • * To ensure Vero cell line safety for biological production.

Main Methods:

  • * Retrovirus detection using reverse transcriptase assay, electron microscopy, and DNA hybridization.

Related Experiment Videos

  • * Tumorigenicity assessment through inoculation of Vero cells into nude mice.
  • * Soft agarose colony formation assay to evaluate cell growth potential.

    • Main Results:

    • * No retroviruses were detected in Vero cells via multiple sensitive methods.
    • * Vero cells (passage 156) did not induce progressively growing tumors in nude mice.
    • * Vero cells exhibited low efficiency of growth in soft agarose, indicating limited tumorigenic potential.

    Conclusions:

    • * The Vero cell line is free from detectable retroviruses.
    • * Vero cells demonstrate a lack of tumorigenic potential.
    • * These findings support the continued use of Vero cells in safe biological production.