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Electrochemiluminescence aptasensor for multiple determination of Hg2+ and Pb2+ ions by using the MIL-53(Al)@CdTe-PEI
Defen Feng1, Penghui Li1, Xuecai Tan1
1School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Key Laboratory of Guangxi Colleges and Universities for Food Safety and Pharmaceutical Analytical Chemistry, Nanning, 530008, China.
Abstract:
An aptasensor based on MIL-53(Al)@CdTe was designed for multiple determination of Hg2+ and Pb2+ by electrochemiluminescence (ECL). Upon the recognition of Hg2+, aptamer 2-AuNPs form hairpin structures and are removed from the electrode. While in the presence of Pb2+, aptamer 1-PtNPs capture the target ions and form G-quadruplexes, and then bring PtNPs close enough to CdTe QDs to produce ECL resonance energy transfer. Upon aptamer interaction with Hg2+ and Pb2+, decreased ECL intensity was observed due to enhanced resonance energy transfer (ERET) and attenuated surface plasmon resonance (SPR). The ECL intensity difference (ΔECL) could therefore be used to detect heavy-metal ions with detection limits of 4.1 × 10-12 M (path 1, Hg2+), 3.7 × 10-11 M (path 2, Pb2+), and 2.4 × 10-11 M (path 3, Pb2+). The aptasensor could also be used for detecting Hg2+ and Pb2+ in fish and shrimp samples with good recoveries.
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