The SPE-assisted europium (III) based complex fluorometric assay for the highly selective and sensitive detection of
1School of Biological Engineering, Dalian Polytechnic University, No.1 Qinggong Road, Ganjingzi District, Dalian, Liaoning, 116034, PR China; Department of Instrumentation and Analytical Chemistry, Key Lab of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, Liaoning, 116023, PR China.
Abstract:
Detection of trace manganese (Ⅱ) ion (Mn2+) is crucial to water safety. Here, commercially available PS-DVB microspheres were sulfonated and then filled into the SPE column in order to separate Mn2+ from complex matrices. Meanwhile, europium (III) complex was prepared with a simple "one pot" method, and its fluorescence intensity was quenched gradually with the increase of Mn2+ concentration. Europium (III) complex combined with home-made SPE column was utilized for highly selective and sensitive measurement of Mn2+. The detectable concentrations of Mn2+ can be low as 0.2 μM, which was less than the drinking water guidelines. Consequently, this new method is promising to assess the content of Mn2+ rapidly and accurately in real-world water samples.
Related Concept Videos
Complexometric Titration: Overview
Complexometric Titration: Ligands
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
Extraction: Advanced Methods


