Distribution of histamine in the cockroach brain and visual system: an immunocytochemical and biochemical study

The distribution of histamine-immunoreactivity in the carbodiimide-fixed brain and visual system of the cockroach was revealed immunocytochemically with an antiserum against histamine (HA). Histamine levels were measured with high-pressure liquid chromatography. The results show a widespread distribution of histamine-containing somata and fibers in the brain, particularly in the visual system. The most intense immunolabeling was seen in the retinal photoreceptors and in the first optic ganglion, the lamina, where the short visual fibers make synaptic connections with the monopolar neurons, which also displayed immunofluorescence. Immunoreactive long visual fibers traversed the lamina and outer chiasma, terminating in the distal medulla. Tracts of histamine-immunopositive fibers appeared to link the optic ganglia to the protocerebrum. Prominent histamine-containing neurons were situated in the lateral protocerebrum. Immunolabeled pathways consisting of large-diameter fibers also were seen in the cockroach brain. The central parts of the brain, including the central body, were reached by thick immunoreactive fibers that gave rise to intensely fluorescent varicose processes there. In the mushroom bodies, immunoreactivity was limited to the calyces. The protocerebral bridge was nonreactive. Immunofluorescence was seen also in the antennal lobes, but not in the antennal nerves. The biochemical measurements correlated well with the immunocytochemical data. The retinas and optic lobes, measured together, contained remarkably large amounts of histamine. These results reinforce the hypothesis presented by Hardie ('87) and Elias and Evans ('83) that histamine functions as a neurotransmitter in the photoreceptors of some, if not all, insect species.