An optimized protocol for high yield expression and purification of an extremophilic protein

Mohamed N Malash¹, Nahla A Hussein², Shaden Muawia³+2

• ¹Microbiology and Immunology Department, Faculty of Pharmacy, Ahram Canadian University, 6th of October City, Giza, Egypt; Biology Department, School of Sciences and Engineering, American University in Cairo, New Cairo, Cairo, Egypt; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Menofia, Egypt.+3