An insight into the dependency on sample preparation for (eco) toxicity assessment of TiO₂ nanoparticles

Assessing the environmental hazard of nanoparticles can be a challenging task using various testing strategies. However, to our knowledge, no information is available about the impact of the sample preparation on the toxicity and toxicity mechanism of nanoparticles. For this aim, three sample preparation methods and their available toxicity procedures were conducted to examine the (eco) toxicity of TiO₂ nanoparticles using bacteria model system. To detail understanding of the effect of sample preparation, the key events on the inhibition were examined by physicochemical and antioxidant responses. The findings showed that the physicochemical and toxicological behavior of the tested TiO₂ NPs varied according to the sample preparation method.