Related Experiment Video

Updated: Dec 29, 2025

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
10:36

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

11.0K

Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures

Karla A Mettrick1, Georgia M Weaver1, Ian Grainge2

  • 1School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2020
PubMed

Keywords:
2-dimensional agarose gelDNA replicationSouthern blottingreplication intermediates

More Related Videos

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

9.5K
Agarose Gel Electrophoresis for the Separation of DNA Fragments07:10

Agarose Gel Electrophoresis for the Separation of DNA Fragments

Published on: April 20, 2012

787.2K

Related Concept Videos

DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
111.4K
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.1K