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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Dec 29, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Next Generation Sequencing (NGS) Application in Multiparameter Gene Expression Analysis.

Degeng Wang^1,2, Andrey L Karamyshev³

¹Department of Environmental Toxicology, Texas Tech University, Lubbock, TX, USA. degeng.wang@ttu.edu.

Methods in Molecular Biology (Clifton, N.J.)

|January 29, 2020

Summary

This summary is machine-generated.

Next generation sequencing (NGS) and RNA-seq are powerful tools for gene expression analysis. Combining RNA-seq with global run-on sequencing (GRO-seq) and polysome profiling allows for genome-wide RNA stability control analysis.

Keywords:

GRO-seq Gene expression regulation Next generation sequencing Nuclear run-on Polysome profiling Polysomes RNA-seq Ribosomes Sucrose gradient Translation

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Last Updated: Dec 29, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Area of Science:

Molecular Biology
Genomics
Toxicology

Background:

Next generation sequencing (NGS) is a standard for gene expression studies.
RNA-sequencing (RNA-seq) offers sensitive, genome-wide RNA quantification.
RNA-seq is applicable to diverse model systems, including environmental toxicology and disease research.

Purpose of the Study:

To describe novel couplings of RNA-seq with other molecular protocols.
To enable genome-wide analysis of RNA stability control mechanisms.

Main Methods:

Coupling RNA-sequencing (RNA-seq) with global run-on sequencing (GRO-seq).
Integrating RNA-seq with polysome profiling via sucrose-gradient-based polysome isolation.
Performing simultaneous RNA-seq, GRO-seq, and polysome profiling analyses.

Main Results:

Genome-wide analysis of RNA stability control was achieved.
The study enabled detailed monitoring of specific gene expression aspects.

Conclusions:

Simultaneous application of RNA-seq, GRO-seq, and polysome profiling provides a comprehensive approach to study RNA biology.
This integrated method advances understanding of gene expression regulation and its role in toxicology and disease.