Related Experiment Video
Updated: Dec 29, 2025
08:43
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Published on: March 10, 2017
10.8K
Ionic fluorescent sensor targeting receptor tyrosine kinases for biosystems imaging and application in flow cytometry
Jiqiu Yin1, Yang Jiao2, Xiaojun Peng2
1State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, 116024, China; College of Medical Laboratory, Dalian Medical University, Dalian, 116044, China.
Biosensors & Bioelectronics
|January 29, 2020
Summary
A novel ionic fluorescent sensor (SNB) enables precise imaging of receptor tyrosine kinases for early cancer diagnosis. This sensor shows potential for advanced diagnostics and treatment monitoring in living biosystems.
Area of Science:
- Biomedical Engineering
- Molecular Imaging
- Cancer Diagnostics
Background:
- Receptor tyrosine kinases (RTKs) are crucial in cancer development and progression.
- Early and accurate detection of RTKs is vital for effective cancer diagnosis and treatment.
- Current imaging techniques for RTKs in living systems face limitations.
Purpose of the Study:
- To design and evaluate a novel ionic fluorescent sensor (SNB) for RTK imaging in living biosystems.
- To assess the sensor's ability to target and image RTKs for early cancer diagnosis.
- To explore the potential of SNB as a versatile tool for various imaging and diagnostic applications.
Main Methods:
- Development of an ionic fluorescent sensor (SNB) integrating sunitinib (targeting unit) and nile blue fluorophore.
- Evaluation of SNB's fluorescence response to RTKs using 2D NMR, optical studies, and kinase activity assays.
- Assessment of SNB's membrane imaging capabilities and selective insertion into RTK pockets on cancer cell lines.
- In vivo application of SNB in flow cytometry for cell sorting and fluorescence imaging in a tumor mouse model.
Main Results:
- The SNB sensor demonstrated distinct fluorescence responses to RTKs, linked to its unfolding strategy and targeting ability.
- SNB exhibited excellent membrane fluorescent imaging via electrostatic adsorption and selective insertion into RTK domain pockets.
- Successful application of SNB in flow cytometry for cell sorting and in vivo fluorescence imaging of tumors in mice.
- The sensor showed high specificity and sensitivity in detecting RTKs in complex biological environments.
Conclusions:
- The designed SNB sensor is effective for fluorescent imaging of RTKs in living biosystems.
- SNB shows significant potential for early cancer diagnosis and monitoring through advanced imaging techniques.
- This technology may become a widely applicable tool for flow cytometry, confocal microscopy, and whole-animal imaging, aiding in cancer treatment.
