Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation of a complementary DNA clone encoding a precursor to human eosinophil major basic protein.

M McGrogan1, C Simonsen, R Scott

  • 1Invitron Corporation, Redwood City, California 94063.

The Journal of Experimental Medicine
|December 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defining a recovery definition and core outcomes of psychosis: protocol of an international Delphi consensus study.

East Asian archives of psychiatry : official journal of the Hong Kong College of Psychiatrists = Dong Ya jing shen ke xue zhi : Xianggang jing shen ke yi xue yuan qi kan·2026
Same author

Desiccation-induced DNA damage facilitates drug resistance in <i>Mycobacterium tuberculosis</i>.

bioRxiv : the preprint server for biology·2025
Same author

Precarious but Possible: A Qualitative Study of the Landscape of Gig Work for People Living with Disabilities and Future Recommendations for Best Practices.

Journal of occupational rehabilitation·2025
Same author

System Dynamics Modeling of Caries Severity States in Long-Term Care.

Journal of dental research·2024
Same author

Pain trajectories and neuropathic pain symptoms following lung cancer surgery: A prospective cohort study.

European journal of pain (London, England)·2024
Same author

Lifetime and point prevalence of psychotic symptoms in adults with bipolar disorders: a systematic review and meta-analysis.

Psychological medicine·2022

Human neutrophils contain a 14-kD protein identical to eosinophil major basic protein (MBP). This study characterizes its cDNA, revealing a 23-kD precursor (pro-MBP) that yields the mature, bipolar MBP.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Human neutrophils (PMNs) possess a 14-kD protein.
  • This protein's N-terminal sequence is identical to eosinophil major basic protein (MBP).

Purpose of the Study:

  • To characterize the human neutrophil 14-kD protein, identified as MBP.
  • To elucidate the structural and functional properties of MBP.

Main Methods:

  • Purification of the 14-kD protein from human PMNs.
  • N-terminal sequencing of the purified protein.
  • Isolation of cDNA clones from an HL-60 cell library.
  • Northern blot analysis of HL-60 cells.
  • Sequence analysis of full-length cDNA clones.

Related Experiment Videos

Main Results:

  • The 14-kD neutrophil protein is identical to eosinophil MBP.
  • MBP is constitutively expressed and highly transcribed in HL-60 cells from a single-copy gene.
  • Full-length cDNA predicts a 23-kD precursor (pro-MBP) cleaved to mature 14-kD MBP.
  • Pro-MBP is a bipolar molecule with distinct hydrophilic (N-terminal) and hydrophobic (C-terminal) halves.

Conclusions:

  • Human neutrophils express MBP, previously thought to be specific to eosinophils.
  • The precursor form, pro-MBP, exhibits a unique bipolar structure.
  • Understanding MBP's structure provides insights into its function in inflammatory responses.