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Related Experiment Videos

Improved static lung preservation with corticosteroids and hypothermia.

T S Hall1, A M Borkon, G C Gurtner

  • 1Johns Hopkins Medical Institutions, Baltimore, Maryland.

The Journal of Heart Transplantation
|September 1, 1988
PubMed
Summary

Methylprednisolone extends lung preservation time to 12 hours in a rabbit model by inhibiting leukocytes. Standard preservation is adequate for 5 hours, but steroid treatment is needed for longer static cold storage.

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Area of Science:

  • Transplantation immunology
  • Organ preservation
  • Surgical research

Background:

  • Extended organ preservation is crucial for successful transplantation.
  • Leukocyte accumulation contributes to lung injury during cold ischemia.
  • Methylprednisolone is a potent inhibitor of leukocyte activity.

Purpose of the Study:

  • To evaluate the efficacy of methylprednisolone in extending static cold preservation of rabbit lungs.
  • To determine the optimal duration for lung preservation with and without methylprednisolone.

Main Methods:

  • Isolated rabbit lung model with static cold preservation at 4°C.
  • Flushing with Euro-Collins' solution and reperfusion with whole blood.
  • Administration of methylprednisolone (30 mg/kg) before harvest and in preservation solutions.

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Main Results:

  • Hypothermia and Euro-Collins' solution provided adequate preservation for up to 5 hours.
  • Methylprednisolone treatment significantly reduced lung edema and injury at 12 hours.
  • Beneficial effects of methylprednisolone were not evident after 24 hours of preservation.

Conclusions:

  • Static lung preservation up to 5 hours is achievable with hypothermia and Euro-Collins' flush.
  • Pharmacologic doses of methylprednisolone can extend static lung preservation to 12 hours.
  • Methylprednisolone ameliorates lung injury during extended cold storage, but efficacy diminishes by 24 hours.