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Serotonergic afferents from the dorsal raphe decrease the excitability of pyramidal neurons in the anterior piriform

Dejuan Wang1, Xiaojie Wang1, Penglai Liu1

  • 1Jiangsu Key Laboratory of Brain Disease and Bioinformation, Research Center for Biochemistry and Molecular Biology, Xuzhou Medical University, 221004 Xuzhou, China.

Proceedings of the National Academy of Sciences of the United States of America
|January 30, 2020
PubMed
Summary

Serotonin (5-HT) reduces the excitability of anterior piriform cortex (aPC) neurons via specific receptors and channels. This finding clarifies how serotonin influences olfactory learning and odor processing in the brain.

Keywords:
anterior piriform cortexelectrophysiologyoptogeneticsserotonergic modulation

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Area of Science:

  • Neuroscience
  • Olfactory System Research
  • Neurotransmitter Modulation

Background:

  • The olfactory system, crucial for behavior and mood, receives significant serotonergic input from the dorsal raphe nucleus.
  • While serotonin's effects on the olfactory bulb are studied, its modulation of the anterior piriform cortex (aPC), vital for olfactory learning and perception, is poorly understood.

Purpose of the Study:

  • To elucidate the mechanism and functional significance of serotonergic modulation in the anterior piriform cortex (aPC).
  • To investigate how serotonin impacts neural activity within the aPC, a key region for olfactory processing.

Main Methods:

  • Utilized a combination of pharmacologic agents, optogenetics, and fiber photometry.
  • Examined serotonergic modulation of neural activity in the aPC both in vitro and in vivo.

Main Results:

  • Serotonin (5-HT) was found to directly decrease the excitability of aPC pyramidal neurons.
  • This reduction in excitability is mediated by 5-HT2C receptors, phospholipase C, and calcium-activated potassium (BK) channels.
  • Endogenous serotonin was observed to attenuate odor-evoked calcium responses in aPC pyramidal neurons.

Conclusions:

  • Identified the specific molecular and cellular mechanisms underlying serotonergic modulation of the anterior piriform cortex.
  • Provided insights into the functional role of serotonin in olfactory learning, odor identity, and intensity encoding.