Deproteinization of four macroporous resins for rapeseed meal polysaccharides

In this study, the adsorption/desorption characteristics of rapeseed meal polysaccharides extract on four resins (HP-20, D3520, XAD-16, and AB-8) were evaluated. The results indicated that HP-20 resin had the best purification effect. Based on static adsorption test, the kinetics and isotherms of the four resins for protein and polysaccharide were investigated. The adsorption test showed that the pseudo-second-order kinetics model and the Freundlich isotherm model were more suitable for explanation of the adsorption process for protein and polysaccharide. Static desorption test showed that the highest protein desorption ratios of HP-20, D3520, and AB-8 resins could be obtained with 60% ethanol solution as eluate, and the highest protein desorption ratios of XAD-16 resin could be obtained with 40% ethanol solution as eluate. Dynamic adsorption/desorption tests of HP-20 resin showed that the deproteinization ratio was 91% and the polysaccharide recovery ratio was 62% when the treatment amount was 1.5 BV. Compared with three traditional methods, HP-20 resin adsorption method that the deproteinization ratio was 82% was more potent than the three traditional methods for purifying polysaccharides from rapeseed meal. In addition, UV/vis spectroscopy showed that most of the protein was absorbed by resins, and FT-IR spectroscopy indicated that the purity of the polysaccharide after purification was improved. Rapeseed meal polysaccharides could be effectively deproteinized using HP-20 resin, and it was suitable for purifying polysaccharides from rapeseed meal.