Seventeen new microsatellites for Tamarix gallica and cross-amplification in Tamarix species
Alejandro Terrones1, Ana Juan1
1Departamento de Ciencias Ambientales y Recursos Naturales Universidad de Alicante Carretera de San Vicente s/n 03690 San Vicente del Raspeig, Alicante Spain.
Premise:
Microsatellite markers were developed for the western Mediterranean tree Tamarix gallica (Tamaricaceae) as part of a study of its genetic diversity and structure.
Methods And Results:
Seventeen microsatellite markers were developed for T. gallica, 14 of which were polymorphic. These microsatellites have di-, tri-, and tetranucleotide repeats with 1-13 alleles per locus and population. Levels of observed and expected heterozygosity ranged from 0.000 to 0.900 and from 0.000 to 0.863, respectively. Six microsatellites showed significant deviations from Hardy-Weinberg equilibrium in at least one population. Cross-amplification in 19 Tamarix species showed a wide transferability to other species of the genus.
Conclusions:
The 14 new polymorphic microsatellite markers will be used to assess the genetic diversity and population genetic structure of T. gallica. Additionally, the successful cross-species amplification suggests their potential usefulness for investigating species delimitation and population genetics in the genus Tamarix.
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