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Updated: Dec 29, 2025

Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
Published on: April 20, 2017
Fusion tags to enhance heterologous protein expression
Mi-Ran Ki1,2, Seung Pil Pack3
1Department of Biotechnology and Bioinformatics, Korea University, 2511 Sejong-Ro, Sejong, 30019, South Korea.
Abstract:
Escherichia coli is the most widely used heterologous protein expression system. However, this system remains a challenge due to the low solubility of proteins, insufficient yield, and inclusion body formation. Numerous approaches have sought to address these issues. The use of a fusion tag is one of the most powerful strategies for obtaining large amounts of heterologous protein in E. coli expression system. Here, recent advances in fusion tags that increase the expression of proteins are reviewed. In addition, proposed concepts for designing peptide tags to increase protein expression are discussed.
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