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Updated: Dec 29, 2025
Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
Published on: November 20, 2013
Impedimetric biosensor fabricated with affinity peptides for sensitive detection of Escherichia coli O157:H7
Fan Shi1, Lingfeng Gan1, Yibing Wang2
1State Key Laboratory of Bioreactor Engineering, Biomedical Nanotechnology Center, Shanghai Collaborative Innovation Center for Biomanufacturing, School of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People's Republic of China.
Objective:
To effectively and conveniently detect pathogenic bacteria, this study aimed to develop label-free biosensors fabricated affinity peptides that can recognize targeted bacteria strains and enable precise quantitative detections.
Results:
A 12-mer peptide with high binding affinity toward Escherichia coli O157:H7 was discovered by biopanning of phage-displayed peptide library. The peptide modified with glycine residues (G3) and one cysteine (C) residue at C-terminal, could self-assemble on gold electrodes, enabling electrochemical impedance spectroscopy (EIS) analysis for quantitative detection of E. coli O157:H7. This method showed a low detection limit of 20 CFU/mL and a liner range from 2 × 102 to 2 × 106 CFU/mL.
Conclusion:
It appears that, by designing and optimizing the structures of peptides, such a strategy can be greatly promising in developing quick, sensitive and quantitative biosensor of pathogens.
