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Updated: Dec 29, 2025

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
Anion exchange chromatography of oligonucleotides under denaturing conditions
Jinyu Sun1, Dhruval Joshi1, Frank Betancourt1
1Department of Chemistry, Brock University, St. Catharines, Canada.
Abstract:
Denaturing anion exchange HPLC simplifies the chromatographic profiles of self-complementary sequences such as d(CG)6, d(CG)6 with locked nucleic acid modifications, d(AT)15, and polymerase chain reaction mixtures. These chromatographic conditions use eluents containing up to 4 M urea at pH 12.4, and lead to the abolishment of secondary structures and meaningful chromatographic patterns of self-complementary sequences. Similarly, PCR template, FAM-labelled primer and FAM-labelled PCR products were resolved, making interpretation of PCR reaction products possible.
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