Primates: Their use in research on vasectomy

Studies based on experimental vasectomies clearly reveal marked species differences in response to vasectomy. In rats, vasectomy invariably results in granuloma formation at the surgical site. In rabbits, immune-complex orchitis develops. Allergic orchitis may also develop in guinea pigs, and the morphological lesions can be adoptively transferred. My co-workers and I have been able to study systematically, biochemically, hormonally, pathologically, and immunologically, primates vasectomized up to 14 years earlier and to compare them with age-matched controls. We have monitored antibody levels in vasectomized rhesus and cynomolgus macaques by sperm-agglutination, sperm-immobilization, and indirect immunofluorescence techniques. Antibodies develop in almost every monkey, in some as early as 10 days after vasectomy. About 50% retain such circulating antisperm antibodies. In men, antibody development is less rapid, and about half of vasectomized men reveal detectable levels. Testicular histopathological studies have revealed detectable levels. Testicular histopathological studies have revealed orchitis, aspermatogenesis, or both, resembling allergic orchitis in most of the vasectomized monkeys and in about one-fourth of the controls. Limited studies of human material reveal some testicular changes. Epididymitis and epididymal granuloma occur exclusively in the vasectomized animals. Use of immunofluorescence has revealed significantly more granular deposits of IgA, IgG, and/or C3 in the basal lamina of the ductus efferens and the caput epididymidis of the vasectomized monkeys. Similar studies on human material have not been done. Both cynomolgus and rhesus macaques have proved to be excellent models for research in atherosclerosis and so have been used to determine whether constant sperm antigen leakage causes immune-complex formation that might result in arteritis and atherosclerosis. Evaluation of the cardiovascular systems from such animals has revealed that vasectomized monkeys have more frequent, more extensive, and more severe arteriosclerosis than age-matched controls. Epidemiological studies are currently under way to determine whether such an effect also occurs in men after vasectomy.