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Tumour-specific microRNA expression pattern in canine intestinal T-cell-lymphomas
Diana Joos1, Miriam Leipig-Rudolph2,3, Karin Weber1
1Clinic of Small Animal Medicine, Centre for Clinical Veterinary Medicine, Ludwig Maximilian University, Munich, Germany.
Veterinary and Comparative Oncology
|January 31, 2020
Summary
MicroRNA expression patterns can distinguish canine intestinal T-cell lymphoma from benign inflammation. This study identified specific down-regulated tumor-suppressing and up-regulated oncogenic microRNAs in lymphoma cases.
Area of Science:
- Veterinary Oncology
- Molecular Diagnostics
- Canine Pathology
Background:
- Canine intestinal T-cell lymphoma diagnosis is challenging due to overlapping enteritis.
- Current diagnostic methods require invasive full-layer biopsies.
- MicroRNAs offer potential for non-invasive diagnostic markers.
Purpose of the Study:
- To characterize microRNA expression in canine intestinal T-cell lymphoma.
- To differentiate lymphoma from lymphoplasmacellular enteritis and healthy tissue.
- To explore microRNA's role in canine lymphomagenesis.
Main Methods:
- Analysis of formalin-fixed, paraffin-embedded canine intestinal tissues.
- RNA extraction, cDNA synthesis, and pre-amplification.
- Quantitative PCR using microRNA arrays and individual assays for 183 canine microRNAs.
Main Results:
- Identified distinct microRNA expression patterns in canine intestinal T-cell lymphoma.
- Down-regulation of tumor-suppressors (miR-194, miR-192, miR-141, miR-203) observed.
- Up-regulation of oncogenic microRNAs (miR-106a~363 cluster) detected; minimal changes in benign conditions.
Conclusions:
- MicroRNA profiling can differentiate canine intestinal T-cell lymphoma from healthy and inflamed tissues.
- Specific microRNA signatures hold diagnostic potential.
- Further understanding of lymphomagenesis through microRNA studies is warranted.
