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Updated: Dec 29, 2025
11:06
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
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Evaluation of acetone as a solvent for the Ames test
Tomomi Shibata1, Takeshi Yamagata2, Akihiro Kawade2
1Management Planning Department, Sunplanet Co., Ltd, 3-5-10 Otsuka, Bunkyo-ku, Tokyo, 112-0012 Japan.
Summary
Acetone is a viable solvent for the Ames preincubation test, even for unstable chemicals. This study confirms its efficacy and safety in bacterial mutagenicity assays.
Area of Science:
- Toxicology
- Genotoxicity Testing
Background:
- Acetone is an alternative solvent for Ames tests when chemicals have poor solubility or stability.
- Limited research exists on acetone's use in the modified Ames test with preincubation.
Purpose of the Study:
- To evaluate acetone as a solvent in the Ames preincubation test.
- To assess acetone's cytotoxicity and impact on mutagenicity detection.
Main Methods:
- Tested 14 mutagens dissolved in acetone (25 or 50 μL) using OECD test guideline 471 bacterial strains.
- Compared results with dimethyl sulfoxide (DMSO) as a solvent (100 μL).
- Assessed acetone's cytotoxicity in bacterial strains with and without S9 mix.
Main Results:
- Acetone showed moderate cytotoxicity in TA1537 strain, similar to DMSO.
- Other strains exhibited mild cytotoxicity with or without S9 mix.
- Acetone did not impede mutagenicity detection; results were comparable or less mutagenic than DMSO.
Conclusions:
- Acetone is a suitable solvent for the Ames preincubation test across five bacterial strains.
- Acetone's use is validated for genotoxicity testing of challenging compounds.
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