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Association between cytokines and two circulating micro-RNAs and development of premature ventricular

Behshid Ghadrdoost1,2, Nahid Aboutaleb3, Mahin Nikougoftar Zarif4

  • 1Physiology Department, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

Iranian Journal of Basic Medical Sciences
|January 31, 2020
PubMed
Abstract

Insights

Inflammation plays a key role in premature ventricular contraction (PVC)-induced cardiomyopathy (PIC). Studies show elevated levels of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in patients with PIC, suggesting their involvement in the disease.

Area of Science:

  • Cardiology
  • Immunology
  • Molecular Biology

Background:

  • Premature ventricular contraction (PVC)-induced cardiomyopathy (PIC) is a condition where frequent PVCs lead to heart muscle dysfunction.
  • Inflammation is increasingly recognized as a significant factor in the development and progression of PIC.

Purpose of the Study:

  • To investigate the expression of key inflammatory markers, including interleukin-6 (IL-6), IL-10, tumor necrosis factor alpha (TNF-α), and interferon-γ (IFN-γ).
  • To evaluate the levels of two specific micro-RNAs, miR-155 and miR-146a, associated with inflammation and cardiovascular disease in patients with PIC.

Main Methods:

  • The study included 25 patients with PIC and 25 controls with normal left ventricular ejection fraction.
  • Inflammatory cytokine and micro-RNA levels were assessed in peripheral blood mononuclear cells (PBMCs) and serum using flow cytometry, real-time PCR, and ELISA.

Main Results:

  • Patients with PIC exhibited significantly higher protein and mRNA expression levels of IL-6 and TNF-α in PBMCs compared to the control group.
  • Circulating levels of IL-6 and TNF-α were also significantly elevated in the PIC group.
  • No significant differences in the expression of miR-155 and miR-146a were observed between the two groups.

Conclusions:

  • Elevated expression of IL-6 and TNF-α in PBMCs of patients with PIC supports their role in the inflammatory pathways underlying this condition.
  • These findings contribute to a better understanding of the pathogenesis of PVC-induced cardiomyopathy.
Keywords:
CardiomyopathiyCytokinesInflammationMicroRNAsPeripheral blood-mononuclear cells

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