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Related Experiment Video

Updated: Dec 29, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Circular RNAs-The Road Less Traveled.

Ashirbad Guria¹, Priyanka Sharma², Sankar Natesan²

¹Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.

Frontiers in Molecular Biosciences

|January 31, 2020

Summary

Circular RNAs (circRNAs), formed by backsplicing, regulate cellular processes by sponging microRNAs. This review explores circRNA biogenesis, function, and detection methods in animals and plants.

Keywords:

backsplicing biogenesis circRNA long non-coding RNA miRNA sponging

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Last Updated: Dec 29, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Circular RNAs (circRNAs) are a recently recognized class of non-coding RNAs.
They are formed through backsplicing of pre-mRNAs during transcription.
circRNAs play roles in regulating gene expression and cellular functions.

Purpose of the Study:

To review the biogenesis, structure, and properties of circRNAs.
To discuss degradation mechanisms and detection methodologies for circRNAs.
To explore the roles and therapeutic potential of circRNAs in animal and plant systems.

Main Methods:

Literature review of circRNA research.
Analysis of circRNA biogenesis pathways (co-transcriptional and post-transcriptional).
Examination of circRNA-microRNA interactions and regulatory functions.

Main Results:

circRNAs function as microRNA sponges, influencing cellular processes.
They exhibit high stability due to their closed-loop structure.
circRNAs are involved in transcriptional regulation and protein translation, with therapeutic potential.

Conclusions:

circRNAs represent a significant area of ongoing research in molecular biology.
Understanding circRNA mechanisms provides new directions for therapeutic strategies.
Diverse methodologies are employed for circRNA identification and functional analysis.