The Effect of Midazolam on Oxidative Stress and Apoptosis in Preterm Infants

Our study aimed to investigate the effect of caffeine and midazolam on oxidative stress and neuronal apoptosis markers in preterm neonates. Methods: Sixty infants between 27-32 weeks of gestational ages were enrolled for this prospective study. Group 1 included patients (n = 23) receiving only early caffeine treatment; group 2 included patients (n = 22) receiving early caffeine treatment with midazolam. Serum levels of total antioxidant capacity (TAC), total oxidative status (TOS) and oxidative stress index (OSI) as oxidative stress markers and serum levels of caspase-3 as an apoptosis indicator were compared in both groups. Results: TAC levels were found significantly higher (p = 0.015) and caspase-3 levels were significantly lower (p = 0.008) in the serum samples of group 2 after treatment. Conclusion: Our study indicated that the treatment with midazolam and caffeine increased TAC and decreased apoptosis markers in preterm infants.