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Related Experiment Videos

Nutritionally variant streptococci develop ultrastructural abnormalities during experimental endocarditis.

C Frehel1, R Hellio, A C Cremieux

  • 1Département de Biologie Moléculaire, Institut Pasteur, Paris, France.

Microbial Pathogenesis
|April 1, 1988
PubMed
Summary

Nutritionally variant streptococci (NVS) develop abnormal structures and exopolysaccharide in endocarditis vegetations. Antibiotic treatment prevented these bacterial alterations, suggesting nutrient limitation contributes to NVS pathogenicity.

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Area of Science:

  • Microbiology
  • Pathology
  • Infectious Diseases

Background:

  • Nutritionally variant streptococci (NVS) cause a significant proportion of endocarditis cases, often presenting as negative blood cultures.
  • The resistance of NVS to antibiotics in infected tissues is a clinical concern.
  • Understanding NVS behavior within cardiac vegetations is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the ultrastructural changes of NVS within cardiac vegetations during experimental endocarditis.
  • To observe the dynamic alterations of NVS at different stages of the disease.
  • To assess the impact of antibiotic treatment on NVS morphology in vivo.

Main Methods:

  • An experimental rabbit model of endocarditis was established.

Related Experiment Videos

  • Scanning and transmission electron microscopy were employed to examine NVS ultrastructure.
  • Ruthenium red staining was used to detect exopolysaccharide.
  • Bacterial morphology was compared between untreated and antibiotic-treated groups.

    • Main Results:

    • In early endocarditis (day 7), NVS showed typical streptococcal morphology.
    • By day 11 and 18, NVS formed large clusters with abnormal ultrastructural features, indicative of unbalanced growth.
    • Extensive exopolysaccharide surrounding NVS clusters was observed in later stages.
    • Antibiotic treatment with penicillin or vancomycin prevented these bacterial alterations.

    Conclusions:

    • NVS undergo significant ultrastructural changes within cardiac vegetations during endocarditis.
    • These changes, including exopolysaccharide production, may be linked to nutrient limitation in bacterial clusters.
    • Antibiotic intervention can prevent these pathological bacterial adaptations.
    • These findings suggest a potential mechanism for NVS pathogenicity and antibiotic resistance in endocarditis.