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Antiplatelet Effectivity between Aspirin with Honey on Cardiovascular Disease Based on Bleeding Time Taken on Mice
Sake Juli Martina1,2, Logaselvi A P Ramar1, Michael R I Silaban1
1Department of Pharmacology and Therapeutics Faculty of Medicine, Jl. Dr Mansur No. 5 Kampus USU, Medan, Indonesia.
Insights
Honey demonstrates significant antiplatelet effects in mice, comparable to aspirin. This research suggests honey as a potential natural alternative for reducing cardiovascular disease risk.
Area of Science:
- Pharmacology
- Cardiovascular Research
- Natural Products
Background:
- Cardiovascular disease (CVD) prevalence and mortality are rising globally.
- Aspirin reduces CVD mortality but carries adverse side effects.
- Both aspirin and honey possess antiplatelet properties, yet their comparative effects remain unexplored.
Purpose of the Study:
- To investigate the antiplatelet effects of honey.
- To compare the antiplatelet efficacy of honey with aspirin using bleeding time in mice.
Main Methods:
- True experimental design with a post-test only control group.
- 32 male mice were divided into placebo, aspirin, and honey groups.
- Oral administration of suspensions for 12 days, followed by bleeding time measurement and statistical analysis (Shapiro Wilk, Kruskal Wallis, Mann Whitney).
Main Results:
- Mean bleeding times: placebo (102.88s), aspirin (369.38s), honey (304.63s).
- Both aspirin and honey significantly increased bleeding time compared to placebo (p=0.001).
- No significant difference in bleeding time between aspirin and honey groups (p=0.172), indicating comparable antiplatelet activity.
Conclusions:
- Honey exhibits significant antiplatelet effects in mice.
- Honey's antiplatelet activity is comparable to aspirin.
- Further research is warranted to explore honey's potential therapeutic use in human cardiovascular disease.
Background:
The prevalence of cardiovascular disease (CVD) and its mortality continues to increase. Various studies have shown aspirin can reduce CVD mortality but has adverse side effects. Research on a comparison between aspirin and honey has not been done, but both have antiplatelet effects.
Aim:
This study is aimed to prove the antiplatelet effects on honey and compare the antiplatelet effects of aspirin with honey based on the bleeding time in mice.
Methods:
This study is a true experimental design with a post-test only control group using 32 male mice, Double Ditsch Webster, ± 3 months old, the weight of 20-30 g, divided into 4 groups. Consisting of a negative control group (placebo), aspirin and honey. The suspension has given orally for 12 days using the probe. The research was conducted at the Laboratory of Pharmacology Department of Pharmacology and Therapeutics Faculty of Medicine, the University of North Sumatra in September until December 2015. The data collected was bleeding time in mice. Data analysed by Shapiro Wilk test, Kruskal Wallis and Mann Whitney.
Results:
The mean bleeding time was a placebo (102.88 seconds), aspirin (369.38 seconds) and honey (304.63 seconds). Mann Whitney test showed significant results in the aspirin and honey groups against the control group (placebo) with p = 0.001. There were no significant differences in the aspirin group against honey (p = 0.172). Honey has an antiplatelet effect in mice. The mean bleeding time in mice given honey is longer or closer to the mean bleeding time in the aspirin group.
Conclusion:
The results could be used as a basis for further research to determine its use in humans with cardiovascular disease.
