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Updated: Sep 14, 2026
Detecting Glycogen in Peripheral Blood Mononuclear Cells with Periodic Acid Schiff Staining
Published on: December 23, 2014
Glycogen Accumulation in Neutrophil Can Be a Marker of Sepsis
Amit Ghosh1, Pranati Nanda1, Swagata Tripathy2
1Department of Physiology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India.
Background:
Sepsis, a systemic inflammatory reaction to infection, is the leading cause of death in the world. The early detection and identification of pro-inflammatory changes in overall metabolism and functioning can help in the proper intervention and control of the inflammatory state, and it will improve the prognosis.
Aim:
In a resource-limited setting where the biomarkers are not easily accessible, this simple technique is required that can help in the early identification of infection and inflammation.
Methodology:
The present study was conducted to find the change in glycogen accumulation and morphological changes during inflammation by preparing a peripheral smear and periodic acid-Schiff (PAS) staining.
Results:
This study shows that the neutrophil accumulates glycogen granules throughout the cytoplasm with the presence of vacuoles in the cytoplasm, thus increasing the neutrophil size and chromatin dispersion.
Conclusion:
PAS staining can be used as a diagnostic method to detect sepsis.
